Skip to main content

Table 1 Sequences of primers and fluorogenic probes used in real time quantitative RT-qPCR analysis (sequences 5′ to 3′)

From: TSC-22 up-regulates collagen 3a1 gene expression in the rat heart

Gene Forward Primer Reverse Primer Fluorogenic Probe
TSC-22 GCAGGAGAACAATC TGCAGCTGGG CCAGTCCGGAG
TGTTGAAGAC CCTGAAAC CAGCTCGCC
ANP GAAAAGCAAACTG CCTACCCCCG TCGCTGGCCCT
AGGGCTCTG AAGCAGCT CGGAGCCT
BNP TGGGCAGAAGATAG ACAACCTCAG CGGCGCAGTCA
ACCGGA CCCGTCACAG GTCGCTTGG
IL-6 ATATGTTCTCAGGG TGCATCATCGC CAGAATTGCCATTGCA
AGATCTTGGAA TGTTCATACAA CAACTCTTTTCTCA
CTGF CGCCAACCGCAAGA CACGGACCCA CACTGCCAAAGA
TTG CCGAAGAC TGGTGCACCCTG
PAI-1 GCTGACCACAGCAG GTGCCCCTCTCA CCCGGCAGCAGAT
GGAAA CTGATATTGAA CCAAGATGCTAT
col1a1 CCCCTTGGTCTTGG GCACGGAAAC CTTTGCTTCCCAGATGT
AGGAA TCCAGCTGAT CCTATGGCTATGATG
Fn-1 GCGAGGCAGGATC CCAATCTTGTA ACCATTGCAAAT
AGCTG GGACTGACCCC CGCTGCCATGAA
Bax2 TCCCCCCGAGAGG CCCAGTTGAAG CCGGGTGGCAGCT
TCTTCT TTGCCATCA GACATGTTTG
Bcl2A GGAACTATATGG AGGCTGAGCA CGACCTCTGTTTG
CCCCAGCAT GGGTCTTCAG ATTTCTCCTGGCTGTC
col3a1 AGCTGGCCTTCCTC GCTGTTTTTGCAG TTCCAGCCGG
AGACTTC TGGTATGTAATG GCCTCCCAG
18S TGGTTGCAAAGCT AGTCAAATTA CCTGGTGGTGC
GAAACTTAAAG AGCCGCAGGC CCTTCCGTCA
  1. TSC-22 transforming growth factor beta-stimulated clone 22; ANP atrial natriuretic peptide; BNP B-type natriuretic peptide; IL-6 interleukin-6; CTGF connective tissue growth factor; PAI-1 plasminogen activator inhibitor-1; col1a1 collagen type Iα1; Fn-1 fibronectin-1; Bax2 Bcl-2-associated X protein; Bcl2A B-cell lymphoma 2A; col3a1 collagen type IIIα1