Table 1 Sequences of primers and fluorogenic probes used in real time quantitative RT-qPCR analysis (sequences 5′ to 3′)
From: TSC-22 up-regulates collagen 3a1 gene expression in the rat heart
Gene | Forward Primer | Reverse Primer | Fluorogenic Probe |
|---|---|---|---|
TSC-22 | GCAGGAGAACAATC | TGCAGCTGGG | CCAGTCCGGAG |
TGTTGAAGAC | CCTGAAAC | CAGCTCGCC | |
ANP | GAAAAGCAAACTG | CCTACCCCCG | TCGCTGGCCCT |
AGGGCTCTG | AAGCAGCT | CGGAGCCT | |
BNP | TGGGCAGAAGATAG | ACAACCTCAG | CGGCGCAGTCA |
ACCGGA | CCCGTCACAG | GTCGCTTGG | |
IL-6 | ATATGTTCTCAGGG | TGCATCATCGC | CAGAATTGCCATTGCA |
AGATCTTGGAA | TGTTCATACAA | CAACTCTTTTCTCA | |
CTGF | CGCCAACCGCAAGA | CACGGACCCA | CACTGCCAAAGA |
TTG | CCGAAGAC | TGGTGCACCCTG | |
PAI-1 | GCTGACCACAGCAG | GTGCCCCTCTCA | CCCGGCAGCAGAT |
GGAAA | CTGATATTGAA | CCAAGATGCTAT | |
col1a1 | CCCCTTGGTCTTGG | GCACGGAAAC | CTTTGCTTCCCAGATGT |
AGGAA | TCCAGCTGAT | CCTATGGCTATGATG | |
Fn-1 | GCGAGGCAGGATC | CCAATCTTGTA | ACCATTGCAAAT |
AGCTG | GGACTGACCCC | CGCTGCCATGAA | |
Bax2 | TCCCCCCGAGAGG | CCCAGTTGAAG | CCGGGTGGCAGCT |
TCTTCT | TTGCCATCA | GACATGTTTG | |
Bcl2A | GGAACTATATGG | AGGCTGAGCA | CGACCTCTGTTTG |
CCCCAGCAT | GGGTCTTCAG | ATTTCTCCTGGCTGTC | |
col3a1 | AGCTGGCCTTCCTC | GCTGTTTTTGCAG | TTCCAGCCGG |
AGACTTC | TGGTATGTAATG | GCCTCCCAG | |
18S | TGGTTGCAAAGCT | AGTCAAATTA | CCTGGTGGTGC |
GAAACTTAAAG | AGCCGCAGGC | CCTTCCGTCA |