Fig. 5

Effects of transforming growth factor beta-stimulated clone 22 (TSC-22) overexpression in cultured neonatal rat ventricular myocytes (NRVMs). TSC-22 a) mRNA levels measured by RT-PCR (n = 8–12) or b) total protein levels determined by Western blot analysis (n = 4) from cultured NRVMs transduced with TSC-22 or LacZ adenovirus for 48 h at the virus amount of 2 MOI, 4 MOI and 8 MOI. **P <0.01, ***P <0.001 versus LacZ (Student’s t-test). The results are expressed as mean ± SEM. Representative Western blots are shown