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Figure 6 | BMC Cardiovascular Disorders

Figure 6

From: Inhibition of S6K1 accounts partially for the anti-inflammatory effects of the arginase inhibitor L-norvaline

Figure 6

Effects of silencing S6K1 on expression of VCAM-1, ICAM-1, and E-selectin. HUVECs were transduced with recombinant adenovirus expressing shRNA against LacZ as control (lanes 1 and 2) or S6K1 (lanes 3 and 4). On day 4 of post transduction, cells were serum-starved for 20 h followed by stimulation with TNFα (10 ng/ml) for 4 hours and extracted. (A) The knocking-down effects of shRNA targeting S6K1 were assessed by immunoblotting for the expression of S6K1 (panel a) and for phosphorylation of its substrate S6 (panel b). Panels c to e reveal the effects on expression of VCAM-1, ICAM-1, and E-selectin. Shown are representative blots from five independent experiments. (B) Quantification of the signals in (A) (panels c – e). All blots were normalized to tubulin expression. Representative data from five independent experiments are reported as mean ± SEM. Values are given as percentage relative to stimulation with TNFα in the control LacZ-shRNA-transduced cells. ***p < 0.001 vs. stimulation with TNFα in the control LacZ-shRNA-transduced cells.

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