Figure 4

Flow cytometry analysis followed by Bax protein expression. H9c2 cells were transfected with pEGFP, pEGFP and pcDNA3-HA- HIF-1α or pcDNA3-Flag-DN-HIF-1α and were sorted in a flow cytometer. The EGFP positive sorted cells were subjected to hypoxia for 6 h. Total Bax expression in the same lysates was used to monitor protein loading. N = Normoxia; H = Hypoxia. The data are representative of 3 separate experiments and only 2 experiments using dominant negative Hif-1α (right panel).