Figure 4

PAI-1 induced levels in fibroblasts with different WRN genotypes. Synchronized fibroblasts with different WRN genotypes were treated with TNFα, TGFβ, or insulin. Twenty four hours later, PAI-1 and WRN expression was evaluated by semiquantitative RT-PCR. Expression results from five cultures were grouped as mean ± standard error of arbitrary units, normalized to β-actin expression. ANOVA test was used to determine differences across genotypes * P < 0.01 against 1367CC/1074FF genotype by Tukey post hoc test.