Figure 4

(A) The levels of endothelin ET _B receptor mRNA expression in human internal mammary arteries, examined using real time PCR. (B) Endothelin ET_B receptor protein expression in human internal mammary arteries, examined using Western blot. The arteries were either not cultured or cultured in the absence or presence of the protein kinase C inhibitors Ro-32-0432 or bisindolylmaleimide I. The results are shown as mean values ± S.E.M of six experiments. Endothelin ET_B mRNA levels in cultured and non-cultured arteries were compared using Student's t-test, where P < 0.05 (*) was considered significant.