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Figure 8 | BMC Cardiovascular Disorders

Figure 8

From: A long-term "memory" of HIF induction in response to chronic mild decreased oxygen after oxygen normalization

Figure 8

Effect of the transition from mild hypoxia to normoxia on the ubiquitylation of HIF-α. HUVECs were exposed to mild decreased oxygen as in Figure 1. Whole cell lysates were co-immunoprecipitated against HIF-1α and Western blot against ubiquitin (top panel) and HIF-1α (bottom panel) completed as in Materials and Methods. Densitometric analysis of ubiquitin expression in HUVECs graphed as a percentage of normoxic ubiquitin expression (dashed line). Data represent the mean +/- S.E.M. from three separate experiments with a total sample size of 4–6. Asterisk, P < 0.05; triple asterisk, P < 0.001 as compared to cells incubated in normoxia for three weeks.

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