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Figure 7 | BMC Cardiovascular Disorders

Figure 7

From: A long-term "memory" of HIF induction in response to chronic mild decreased oxygen after oxygen normalization

Figure 7

Effect of various inhibitors and anti-oxidants on "memory" for HIF-1α, HIF-2α, Glut-1, BNIP3, PAI-1, iNOS and VEGF expression in human endothelial cells. HUVECs were exposed to two weeks of mild decreased oxygen followed by one weeks of normoxia (D→N) in the presence or absence of 5 μM of the HIF inhibitor YC-1, or 62.5 μM α-lipoic acid (ALA), or 25–100 PFU/cell UCP2 adenovirus, or 10 μM apocynin (APO), or 10 μM oxypurinol (OXY) or to 1 mM L-NAME. Western blots were performed against HIF-1α (A), HIF-2α (B), BNIP3 (D), PAI-1 (E), iNOS (F) and GLUT-1 (G) on cellular lysates and ELISA was performed against VEGF (C) on cellular supernatants. Graphs for each Western blot indicate the densitometric analysis relative to the respective "memory" (D→N) protein expression (dashed line). Data in graphs represent the mean +/- S.E.M. from three separate experiments with a total sample size of 4–6 and with representative gels above each graph. Double asterisk, P < 0.01; triple asterisk, P < 0.001 as compared to cells in "memory" (D→N) treatment.

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