Figure 2

Effect of chronic relative hypoxia on a marker of cell proliferation in HMEC-1 cells. HMEC-1 cells were exposed to normoxia (20% O2) or to various levels of relative hypoxia for 3 weeks followed by a one hour incubation with 5 μM CFDA-AM and fluorescence measurement as in methods. The data represent the mean +/- SEM of triplicate wells from 3 independent experiments. ** = P < 0.01 and *** = P < 0.001 using one-way ANOVA with a Neuman-Keuls multiple comparisons post-test.