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Table 1 Explant Success by Experimental Variables

From: A cell culture model using rat coronary artery adventitial fibroblasts to measure collagen production

  Type of Buffer
  PBS HBS Kreb's
Percent of successful explants 40–50% 80–90% 80–90%
  Time from Tissue Harvest to Culture
  < 1 hr 1–2 hrs > 2 hrs
Percent of successful explants 80–90% 50–60% 0–5%
  Age of Animal
  10–12 wks 18 wks > 30 wks
Percent of successful explants 80–90% 25–30% 0%