Table 1 Explant Success by Experimental Variables
Type of Buffer | |||
|---|---|---|---|
PBS | HBS | Kreb's | |
Percent of successful explants | 40–50% | 80–90% | 80–90% |
Time from Tissue Harvest to Culture | |||
< 1 hr | 1–2 hrs | > 2 hrs | |
Percent of successful explants | 80–90% | 50–60% | 0–5% |
Age of Animal | |||
10–12 wks | 18 wks | > 30 wks | |
Percent of successful explants | 80–90% | 25–30% | 0% |