Figure 4
Collagen Type I (μg) measured by ELISA in LAD fibroblasts treated with 10-7M testosterone or 10-7M estrogen. Testosterone significantly increased the amount of collagen deposited (2.912 ± 0.247 μg) compared to control values (2.475 ± 0.211 μg) (* p < 0.05, 1 tailed t-test). Estrogen decreased the amount of collagen (2.103 ± 0.262 μg) compared to control (p = 0.058, 1 tailed t-test). Collagen was measured in wells with a 0.32 cm2 growth area from a 96 well culture plate.