Figure 7

Plasma levels of adiponectin and TNFα. (A) Immunoblot analysis shows the levels of adiponectin in the plasma. Blood sample from both control and SSW smoking mice were applied to 7.5% polyacrylamide electrophoresis gel and transferred to the nitrocellulose membrane. Adiponectin was probed with a goat anti-mouse adiponectin antibody and with a secondary conjugated to HRP for subsequent use with the enhanced chemiluminescence system (ECL, Amersham Pharmacia). Less adiponectin was detected in the plasma of mice exposed to SSW. Samples representative of 2 animals per group. (B) Cytokine array results showing TNFα levels in the plasma. At early stages of smoking, there was no significant difference between the TNFα levels in the control and SSW smoked mice, whereas at late stages of SSW smoking, the TNFα level in plasma was significantly increased. This increment was correlated with the decrease of adiponectin and increment of MCP-1 in the plasma. Samples were done in triplicate.