Figure 4

Lipid accumulation in liver tissue. About equal amounts of snap-frozen liver from the sacrificed mice of both control and SSW mice were used for lipid extraction with chloroform: methanol (2:1). The extracted lipids were dried and redissolved in 1% Triton X-100. Commercial kits (WAKO Chemicals USA, Inc., Richmond, VA) were used for determination of (A) total cholesterol and (B) triglycerides. (C-F) Oil Red O staining of frozen liver sections reveals the lipid contents in the liver of control mice (C) and SSW mice (D). The liver of control mice (E) and SSW mice (F) were also stained with Diff-Quick to show their histological structure. The mice were exposed to SSW for 1 year. Representative images of one mouse out of three.