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Figure 2 | BMC Cardiovascular Disorders

Figure 2

From: The effects of second-hand smoke on biological processes important in atherogenesis

Figure 2

Effect of cigarette smoke on the HDL and its ratio to other plasma lipids. Mice were exposed to SSW 6 hrs/day intermittently, 5 days a week. Blood samples were collected from the tail vein and, after centrifugation, the plasma was used to analyze for levels of HDL and other lipids by enzymatic reaction using colorimetric methods with commercially available kits (Wako Chemicals). After incubating with coloring reagent that only reacts with a specific cholesterol or type of lipid, the absorbance was measured and the concentration of each was calculated by plotting the measured values against a standard curve. (A) The levels of HDL in the blood of mice exposed to SSW are significantly lower than in the non-exposed mice. The ratios of HDL to LDL (B), HDL to TG (C), and HDL to TC (D) are all significantly lower in the SSW exposed mice.

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