Figure 1

Detection of hCAP-18/LL-37 by immunohistochemistry in sections of human aorta. (A) Low power view. Binding of the specific antibody was detected by peroxidase, which yields a brown color reaction against a pale background. Dense binding was found in the neointima (N) of an athreosclerotic lesion. The vascular lumen (L) and the tunica muscularis (TM) are indicated. (bar, 100 μm). (B) Portion of the neointima indicated in (A). hCAP-18/LL-37-like immunoreactivity is found (bar, 100 μm). (C) Control, where the specific antibody has been replaced by nonimmune serum, resulting in loss of labeling (bar, 100 μm). (D) High power image of a slide double stained for hCAP-18/LL-37 (brown) and the macrophage marker CB68 (red). HCAP-18/LL-37 and CD68 immunoreactivity are mainly co-localized to the same cells (bar, 10 μm).