Figure 4

ERα expression in HCAEC. The Western blots depict ERα levels in HCAEC, isolated from a male (M) and a female (F) donor prior to initiation of ER ligand incubation as well as after completion of 72 h incubation in the absence (CNT) or presence of 10 nM each of E2, EE2, tamoxifen (TAM), raloxifene (RAL) or fulvestrant (FUL). A whole cell lysate of MCF7 cells was included as positive control and expression of α-tubulin was measured as loading control. Normal non-specific mouse IgG and a blocking peptide was included to ensure specificity of the bands.