Figure 4

Multi-electrode array recording in co-cultures of primary skeletal myoblasts with cardiac myocytes. Microscopic images of the multi-electrode array (MEA) dishes containing co-cultures of cardiac myocytes with non-transduced (A) or connexin 43/EGFP transduced (C) primary skeletal myoblasts. The images were taken under UV light with 100 times magnification. Skeletal myoblasts transduced with MLV-CX43-EGFP vector expressed the EGFP marker and, therefore, were fluorescent. Individual recordings (x-axis – time in s, y-axis – potential in μV) from the electrode No. 33 of the MEA in co-culture of cardiac myocytes with non-transduced skeletal myoblasts (B) and in co-culture of cardiac myocytes with connexin 43/EGFP transduced skeletal myoblasts (D). These electrograms show the last stimulatory pulse in the series of 10 and the first resultant FAP (D) or absence of it (B). Ten stimulatory current pulses were applied with the frequency of 1 Hz.