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Figure 3 | BMC Cardiovascular Disorders

Figure 3

From: Overexpression of connexin 43 using a retroviral vector improves electrical coupling of skeletal myoblasts with cardiac myocytes in vitro

Figure 3

Intercellular fluorescent dye microinjection in connexin 43 and EGFP transduced and non-transduced primary skeletal myoblasts. Cascade Blue derivative 8-methoxypyrene-trisulphonic acid was used as a fluorescent dye. Panel 'ABCD' shows fluorescent dye microinjection experiment in a culture of non-transduced primary skeletal myoblasts. Panel 'EFGH' shows fluorescent dye microinjection experiment in a culture of connexin 43 and EGFP transduced primary skeletal myoblasts. All pictures were taken using 400 times instrumental magnification. Blue fluorescence was visualised using Nikon filter block UV-1A (DM400). Green fluorescence was visualised using a standard 'FITC' filter block. (A) A phase contrast image of a group of adjacent non-transduced skeletal myoblasts. (B) An image of the injection microelectrode inserted into one of the adjacent cells under UV light. (C) An image of the same group of the cells under UV light after 2 min, no dye transfer. (D) An overlay of 'A' and 'C'. (E) An image of a group of adjacent connexin 43 and EGFP transduced skeletal myoblasts under UV light before dye injection (green fluorescence confirms EGFP expression). (F) An image of the injection microelectrode inserted into one of the adjacent cells under UV light. (G) An image of the same group of cells under UV light after 2 min, the dye is transferred to adjacent cells. (H) An overlay of 'E' and 'G'.

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