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Table 6 Constitutively active ALK1 regulated gene expression in primary HUVECs and the HUVEC cell line ECRF24. ECRF24 cells were infected with an MOI of ~50 with the recombinant adenovirus AdLacZ, containing the LacZ gene, or AdALK1QD, respectively. Primary HUVECs were infected with an MOI of ~50 with the empty AdCMV3 adenovirus or AdALK1QD, respectively. Gene expression was assessed after 24 hours by qRT-PCR. Non-infected cells served as reference. qRT-PCR based relative gene expression was calculated using the comparative ΔΔCT method. RQ represents the relative expression level (fold change) compared to the reference sample. RQMax/Min are the relative maximum/minimum expression levels of the mean expression level RQ. The right column titled HMEC-1 shows in comparison the mean RQ level of three independent experiments with HMEC-1 cells infected with AdALK1QD (see Table 4). Genes with RQ values in bold are considered to be up-regulated.

From: ALK1 signalling analysis identifies angiogenesis related genes and reveals disparity between TGF-β and constitutively active receptor induced gene expression

  ECRF24 primary HUVECs HMEC-1
Gene [GenBank number] AdLacZ RQ (RQMax/Min) AdALK1QDRQ (RQMax/Min) AdCMV3 RQ (RQMax/Min) AdALK1QDRQ (RQMax/Min) AdALK1QDRQ
GADD153 [S40706] 0.95 (1.07/0.85) 4.21 (5.27/3.37) 1.17 (1.27/1.07) 7.53 (12.05/4.70) 4.55
IL-8 [M28130] 1.13 (1.01/1.27) 11.55 (13.60/9.81) 1.52 (2.55/0.90) 4.37 (5.57/3.44) 1056.07
SMAD6 [U59914] 0.82 (0.90/0.75) 5.65 (6.59/4.84) 1.30 (1.44/1.17) 38.89 (44.26/34.18) 14.56
COL5A1 [D90279] 1.04 (1.15/0.94) 1.68 (1.94/1.45) 1.00 (1.10/0.92) 3.47 (4.06/2.96) 2.28
HEF1 [L43821] 0.89 (0.98/0.81) 3.88 (4.55/3.31) 0.61 (0.66/0.56) 1.18 (1.75/0.80) 3.93
TLR4 [U93091] 0.95 (1.06/0.85) 1.59 (1.82/1.39) 1.02 (1.12/0.93) 4.77 (5.70/3.99) 3.98
ET-1 [J05008] 0.64 (0.70/0.59) 0.73 (0.86/0.61) 1.07 (1.16/0.99) 2.21 (2.62/1.87) 3.87
CARP [X83703] 0.61 (0.74/0.50) 0.79 (1.08/0.58) 0.83 (1.00/0.69) 1.94 (2.82/1.33) 4.29
HPTPη [D37781] 1.69 (1.90/1.51) 1.54 (2.75/0.86) # # 2.08
ID1 [S78825] 0.70 (0.78/0.63) 2.06 (2.45/1.73) 1.40 (1.79/1.09) 4.04 (6.63/2.46) 6.56
BMP6 [M60315] 0.64 (0.70/0.57) 0.72 (0.82/0.63) 0.91 (1.15/0.73) 1.61 (2.04/1.27) 4.26
ALK5 [L11695] 0.68 (0.78/0.59) 0.95 (1.25/0.71) 0.41 (0.44/0.38) nd 1.36
TβRII [M85079] 1.18 (1.31/1.06) 0.77 (0.91/0.65) 0.96 (1.04/0.89) 1.38 (1.65/1.16) 1.26
Endoglin [J05481] 0.83 (0.93/0.74) 1.19 (1.60/0.89) 1.02 (1.10/0.95) 3.05 (4.07/2.29) 2.36
c-myc [V00568] 0.85 (1.03/0.70) 0.78 (0.90/0.67) 1.00 (1.17/0.86) 1.05 (1.64/0.67) 1.45
ALK1 [L17075] (ABI assay) 0.95 (1.09/0.83) 0.93 (1.11/0.77) 0.86 (0.93/0.79) 1.44 (2.28/0.91) 1.40
ALK1 [L17075] (own assay) 0.50 597.72* 0.55 197.18* 5079.92*
  1. nd, not detected after 40 cycles; #, not detected after 40 cycles and not detected in the reference sample; *, ectopic expression