ALK1 signalling analysis identifies angiogenesis related genes and reveals disparity between TGF-β and constitutively active receptor induced gene expression

Table 3 The selection of reference samples used for each of the comparative analyses for relative gene expression calculation based on qRT-PCR.

Reference samples:	Test samples:
HMEC-1 + AdCMV3 24 hr; Experiment 1 HMEC-1 + AdCMV3 24 hr; Experiment 2 HMEC-1 + AdCMV3 24 hr; Experiment 3	HMEC-1 + AdALK1^QD 24 hr; Experiment 1 HMEC-1 + AdALK1^QD 24 hr; Experiment 2 HMEC-1 + AdALK1^QD 24 hr; Experiment 3
HMEC-1 + AdCMV3 4 hr; Experiment 1 HMEC-1 + AdCMV3 4 hr; Experiment 2	HMEC-1 + AdALK1^QD 4 hr; Experiment 1 HMEC-1 + AdALK1^QD 4 hr; Experiment 2
HUVEC, no virus	HUVEC + AdCMV3 HUVEC + AdALK1^QD
ECRF24 cells no virus	ECRF24 + AdLacZ ECRF24 + AdALK1^QD
HMEC-1 no TGF-β1, 16 hr incubation	HMEC-1, 0.5 ng/ml TGF-β1, 16 hr incubation HMEC-1, 4.0 ng /mlTGF-β1, 16 hr incubation
HMEC-1 no TGF-β1, 24 hr incubation	HMEC-1, 0.5 ng /mlTGF-β1, 24 hr incubation HMEC-1, 4.0 ng/ml TGF-β1, 24 hr incubation

ISSN: 1471-2261