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Table 3 The selection of reference samples used for each of the comparative analyses for relative gene expression calculation based on qRT-PCR.

From: ALK1 signalling analysis identifies angiogenesis related genes and reveals disparity between TGF-β and constitutively active receptor induced gene expression

Reference samples: Test samples:
HMEC-1 + AdCMV3 24 hr; Experiment 1
HMEC-1 + AdCMV3 24 hr; Experiment 2
HMEC-1 + AdCMV3 24 hr; Experiment 3
HMEC-1 + AdALK1QD 24 hr; Experiment 1
HMEC-1 + AdALK1QD 24 hr; Experiment 2
HMEC-1 + AdALK1QD 24 hr; Experiment 3
HMEC-1 + AdCMV3 4 hr; Experiment 1
HMEC-1 + AdCMV3 4 hr; Experiment 2
HMEC-1 + AdALK1QD 4 hr; Experiment 1
HMEC-1 + AdALK1QD 4 hr; Experiment 2
HUVEC, no virus HUVEC + AdCMV3
HUVEC + AdALK1QD
ECRF24 cells no virus ECRF24 + AdLacZ
ECRF24 + AdALK1QD
HMEC-1 no TGF-β1, 16 hr incubation HMEC-1, 0.5 ng/ml TGF-β1, 16 hr incubation
HMEC-1, 4.0 ng /mlTGF-β1, 16 hr incubation
HMEC-1 no TGF-β1, 24 hr incubation HMEC-1, 0.5 ng /mlTGF-β1, 24 hr incubation
HMEC-1, 4.0 ng/ml TGF-β1, 24 hr incubation