ALK1 signalling analysis identifies angiogenesis related genes and reveals disparity between TGF-β and constitutively active receptor induced gene expression

Table 1 Gene-specific oligonucleotides for sqRT-PCR. Primer sets were designed across exon-exon boundaries to avoid amplification of genomic DNA in the cDNA samples that might contain traces of genomic DNA. ALK1 Primers were also used for qRT-PCR analyses (own assay).

Gene	Forward primer (5' – 3' orientation)	Reverse primer (5' – 3' orientation)	PCR annealing Temp (°C)
TLS	TTGAGTCTGTGGCTGATTAC	CACTGGTTGCATTCATTCCT	56
GADD153	GTCTAAGGCACTGAGCGTAT	TGGGGAATGACCACTCTGTT	58
IL-8	GATTGAGAGTGGACCACACT	TCGGATATTCTCTTGGCCCT	58
SMAD6	GTCTTACACTGAAACGGAGG	AGCTGATGCGGACGCTGTTG	58
COL5A1	TCGATCCTAACCAAGGATGC	TGTGACGCTTCACCGAAGTC	58
HEF1	GAAGAATGGGCCGGAGAGCA	GGGAAATGAAATGGGTCTCA	56
TLR4	ACAAAATCCCCGACAACCTC	ATGTAGAACCCGCAAGTCTG	58
ET-1	CCTGGACATCATTTGGGTCA	AGGGCTTGCCTTTCAGCTTG	58
CARP	ACCGCTATAAGATGATCCGA	AATGAAGCTCTGCTCACCAG	56
HPTPη	GGGCACTTTCATTGCCATTG	CATAGCTCCCTTTTCCTGGT	58
DNAJB1	ACTACTACCAGACGTTGGGC	CCGCTTGTGGGAGATTTTCG	60
MGSA	GGGAATTCACCCCAAGAACA	ATCACAGTGGCTGGCATGTT	58
c-myc	AGGCTATTCTGCCCATTTGG	CCACATACAGTCCTGGATGA	58
ALK1	GCAACCTGCAGTGTTGCATC	CGGATCTGCTCGTCCAGCAC	60
GAPDH	ACCACAGTCCATGCCATCAC	TCCACCACCCTGTTGCTGTA	60

ISSN: 1471-2261