Figure 3

Analysis of SR-BI expression in human macrophages and human carotid atherosclerotic plaque by RT-PCR. Schematic illustration of the human SR-BI cDNA and the location of the primers used for PCR (A). Analysis of expression of SR-BI and SR-BI isoforms in RNA extracted from macrophages by RT-PCR (B). PCR products from four donors (D1–D4) and PCR products from donor 4 digested with Nco I (D4+Nco I), non-template control (ntc), 1 kb DNA-molecular marker (m). Analysis of expression of SR-BI and SR-BI isoforms in RNA extracted from atherosclerotic plaque tissue by RT-PCR (C). PCR products from atherosclerotic plaque tissue cDNA from four patients (P1–P4), PCR product from patient 4 digested with Nco I (P4+Nco I). PCR product corresponding to undigested SR-BI (476 bp), SR-BII (347 bp) and SR-BIII (541 bp) are indicated.