Table 2 Oligonucleotide primers used for real time PCR or rat sequences
Target Gene | Primer Sequence | Length of Amplicon | GenBank™ accession no. |
|---|---|---|---|
Abcg5 | Forward: 5'-AGGCTCAGTTACAGGCTCAGAG-3' Reverse: 5'-GTCCCACTTCTGCTGGCATGAT | 118 bp | AF312714 |
Abcg8 | Forward: 5'-CGTCAGATTTCCAATGACTTCCG-3' Reverse: 5'-TCCGTCCTCCAGTTCATAGTACA-3' | 241 bp | AF351785 |
Cyp7a1 | Forward: 5'-CAGGGAGATGCTCTGTGTTCA-3' Reverse: 5'-AGGCATACATCCCTTCCGTGA-3' | 121 bp | NM_012924 |
HMG-CoA Reductase | Forward: 5'-GAACTGAAACACGGGCATTGG-3' Reverse: 5'-ACGGCACGGAAAGAACCATAG-3' | 189 bp | X55286 |
Srebp-2 | Forward: 5'-TGGAAGGAAGGTAGAGTAGGTGGG-3' Reverse: 5'-TTTTGTGGACTGCTTGGCTCAGGG-3' | 152 bp | AA901364 |
GAPDH | Forward: 5'-TCAAGAAGGTGGTGAAGCAGGC-3' Reverse: 5'-GCATCAAAGGTGGAAGAATGGG-3' | 118 bp | AF106860 |