Figure 8
Protein Expression of Abcg5 in liver total membrane preparations. Membrane preparations (20 μg protein) from rat tissues were isolated and analysed by SDS-PAGE, blotted and probed with anti-Abcg5 antiserum (R116, panel A), probed with anti-transferrin antiserum (panel B) or the gel was fixed and stained with Coomassie Brilliant Blue R-250 (panel C). Lane 1 contains a recombinant E. coli expressed N-terminal fragment of sterolin-1 as a control for antibody binding, lanes 2 and 3 contain Sprague-Dawley (SD) spleen membrane extracts. Liver from Wistar (lanes 4 and 5), SHR (lanes 6 and 7) and Sprague-Dawley (SD, lanes 8 and 9) Densitometry measurements when normalised to transferrin show a 2-fold increase in sterolin-1 protein in SHR rat liver samples, compared to SD and Wistar rat liver. No antibody for sterolin-2 is currently available.