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Figure 23 | BMC Cardiovascular Disorders

Figure 23

From: Angiotensin II induced inflammation in the kidney and in the heart of double transgenic rats

Figure 23

Effects of PDTC on the activation of DNA binding nuclear factors in the kidney (A) and heart (D). EMSA for the detection of NF-κB shows a higher binding activity of dTGR kidney and heart homogenates compared with SD rats. NF-κB DNA binding activity in the kidney was markedly reduced by PDTC treatment and even more in the heart. Each lane represents a separate animal. Renal homogenates were incubated with antibodies against the NF-κB subunits anti-p50, anti-p65, anti-c-Rel, and anti-Rel B (B). Specificity of binding (C) was demonstrated by competition of excess unlabeled oligonucleotides containing the kB site from the MHC-enhancer (H2K). EMSA was performed 3 times independently with similar results.

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