Table 1 Sequences of primers used in qRT-PCR
From: Resistance to cardiomyocyte hypertrophy in ae3 −/− mice, deficient in the AE3 Cl−/HCO3 −exchanger
Target gene | Primer sequence | |
|---|---|---|
Anp | F: | 5′-TCCAGGCCATATTGGAGCAAATCC-3′ |
R: | 5′-TCCAGGTGGTCTAGCAGGTTCTTG-3′ | |
β-mhc | F: | 5′-GAGACGGAGAATGGCAAGAC-3′ |
R: | 5′-AAGCGTAGCGCTCCTTGAG-3′ | |
Caii | F: | 5′-CTCTGCTGGAATGTGTGACCT-3′ |
R: | 5′-GCGTACGGAAATGAGACATCTGC-3′ | |
Nhe1 | F: | 5′-TTTTCACCGTCTTTGTGCAG-3′ |
R: | 5′-TGTGTGGATCTCCTCGTTGA-3′ | |
Gapdh | F: | 5′-CCTCGTCCCGTAGACAAAAT-3′ |
R: | 5′-TGATGGCAACAATCTCCACT-3′ | |