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Figure 7 | BMC Cardiovascular Disorders

Figure 7

From: Resistance to cardiomyocyte hypertrophy in ae3 −/− mice, deficient in the AE3 Cl/HCO3 exchanger

Figure 7

Effect of hypertrophic stimuli on protein synthesis. Cardiomyocytes were isolated from both ae3 −/− and ae3 +/+ (wildtype) adult mice heart. Following 18 h culture period, vehicle (open bars, control), ANGII (1 μM, black bars) and PE (10 μM, blue bars) were added for further 24 h. Radiolabeled phenylalanine ([3H]-Phe, 1 μM), was added immediately after drug intervention and cells were incubated for further 24 h. Cells were harvested and proteins were precipitated by TCA precipitation. Incorporated [3H]-Phe was measured by scintillation counting and expressed relative to the vehicle control group as a percentage. *P < 0.05 compared to control group (n = 5).

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