Figure 6

Effect of hypertrophic stimuli on expression of CAII and NHE1 mRNA. Cardiomyocytes were isolated from ae3 ^−/− and ae3 ^+/+ adult mouse hearts. Following 18 h culture period, vehicle (open bar, control), ANGII (1 μM, black bars) or PE (10 μM, blue bars) were added for further 24 h. To determine the mRNA expression levels of NHE1 or CAII, quantitative real-time PCR was performed. RNA prepared from cardiomyocytes was reverse transcribed and the resulting cDNA was subjected to qRT-PCR. Cycle threshold (Ct) value was obtained for NHE1 or CAII and GAPDH. Ct values of each sample were corrected for the respective GAPDH Ct values. Absolute differences in gene expression between samples is based on the relation that a difference of one cycle corresponds to a difference of two-fold in template abundance. Relative transcript abundance was expressed as fold-change of NHE1 (A) or CAII (B) relative to control. *P < 0.05, compared to ae3 ^+/+ control group. # P < 0.05, compared to ae3 ^−/− control group (n = 4).