Figure 5
From: Resistance to cardiomyocyte hypertrophy in ae3 −/− mice, deficient in the AE3 Cl−/HCO3 −exchanger
Effect of hypertrophic stimuli on expression of hypertrophic marker mRNA. Cardiomyocytes were isolated from ae3 −/− and ae3 +/+ adult mouse hearts and maintained in cell culture. Following 18 h culture period, vehicle alone (control, open bars), ANGII (1 μM, black bars) or PE (10 μM, blue bars) were added for further 24 h. To determine the mRNA expression levels of ANP or β-MHC, quantitative real-time PCR was performed. RNA prepared from cardiomyocytes was reverse transcribed and the resulting cDNA was subjected to qRT-PCR. Cycle threshold (Ct) value was obtained for ANP or β-MHC and GAPDH. Ct values of each sample were corrected for the respective GAPDH Ct values. Absolute differences in gene expression between samples is based on the relation that a difference of one cycle corresponds to a difference of two-fold in template abundance. Relative transcript abundance was expressed as fold-change of ANP (A) or β-MHC (B) relative to control. *P < 0.05, compared to control group (n = 4).