Figure 1
From: Manipulation of valve composition to elucidate the role of collagen in aortic valve calcification
Leaflet characterization immediately after collagenase treatment, prior to in vitro organ culture. Quantification of (A) total collagen, (B) HA, (C) total sGAGs, (D) DNA, (E) swelling ratio, and (F) Young’s Modulus indicated that collagenase-treated leaflets were selectively depleted of collagen. (G) Movat’s Pentachrome staining indicated depletion of collagen (yellow) in collagenase-treated leaflets, but retention of other valve components, such as proteoglycans (blue). Scale bar = 400 μm. *P <0.05 compared to undepleted leaflets (no enzyme).