Table 1 RNA yield and quality before and after DNase I treatment
Samples before Dnase I treatment | OD260 | OD280 | OD260/ OD280 | RNA concentration(ng/μl) |
|---|---|---|---|---|
IVBD-1 | 17.079 | 8.765 | 1.95 | 683.14 |
IVBD-2 | 15.494 | 7.962 | 1.95 | 619.77 |
IVBD-3 | 5.603 | 3.829 | 1.46 | 224.11 |
IVBD-4 | 11.815 | 5.889 | 2.01 | 472.58 |
IVBD-5 | 17.626 | 8.540 | 2.06 | 705.05 |
Control-1 | 13.908 | 7.215 | 1.93 | 556.32 |
Control-2 | 9.294 | 4.841 | 1.92 | 371.75 |
Control-3 | 11.153 | 5.965 | 1.87 | 446.14 |
Control-4 | 14.234 | 7.344 | 1.94 | 569.36 |
Control-5 | 14.797 | 7.684 | 1.93 | 591.89 |
Samples after DNase I treatment | OD 260 | OD 280 | OD 260 / OD 280 | RNA concentration(ng/ul) |
IVBD-1 | 7.994 | 3.939 | 2.03 | 319.76 |
IVBD-2 | 6.840 | 3.493 | 1.96 | 273.59 |
IVBD-3 | 5.417 | 2.769 | 1.96 | 216.68 |
IVBD-4 | 8.579 | 4.371 | 1.96 | 343.16 |
IVBD-5 | 12.352 | 6.073 | 2.03 | 494.08 |
Control-1 | 4.455 | 2.307 | 1.93 | 178.20 |
Control-2 | 4.807 | 2.405 | 2.00 | 192.27 |
Control-3 | 10.141 | 5.244 | 1.93 | 405.63 |
Control-4 | 10.424 | 5.408 | 1.93 | 416.96 |