Overexpression of BRG1 modulates aortic SMCs from contractile to synthetic phenotype. Aortic SMCs were infected with Ad.BRG1 (or Ad.Null) at a MOI of 100. (A, B) The expression level of α-SMA mRNA and protein was detected at 72 hr post-infection.by qRT-PCR and western blot and normalized to that of GAPDH and β-actin, respectively. Histogram showed the expression level of α-SMA mRNA (A) and protein (B) in Ad.BRG1-infected and Ad.Null-infected aortic SMCs. (C) The percentage of α-SMAhigh aortic SMCs were analyzed by flow cytometry. Flow cytometric contour plots showed the percentage of stained cells. Histogram showed the percentage of α-SMAhigh cells of Ad.BRG1-infected and Ad.Null-infected aortic SMCs. Data represent mean ± SEM from 4 independent experiments; *, P <0.05 by t test.**, P <0.01 by t test.