Figure 7
From: BRG1 overexpression in smooth muscle cells promotes the development of thoracic aortic dissection
Overexpression of BRG1 modulates aortic SMCs from contractile to synthetic phenotype. Aortic SMCs were infected with Ad.BRG1 (or Ad.Null) at a MOI of 100. (A, B) The expression level of α-SMA mRNA and protein was detected at 72 hr post-infection.by qRT-PCR and western blot and normalized to that of GAPDH and β-actin, respectively. Histogram showed the expression level of α-SMA mRNA (A) and protein (B) in Ad.BRG1-infected and Ad.Null-infected aortic SMCs. (C) The percentage of α-SMAhigh aortic SMCs were analyzed by flow cytometry. Flow cytometric contour plots showed the percentage of stained cells. Histogram showed the percentage of α-SMAhigh cells of Ad.BRG1-infected and Ad.Null-infected aortic SMCs. Data represent mean ± SEM from 4 independent experiments; *, P <0.05 by t test.**, P <0.01 by t test.