Overexpression of BRG1 induces apoptosis of aortic SMCs. Aortic SMCs were infected with Ad.BRG1 (or Ad.Null) at a MOI of 100. The expression of BRG1 and its effect on cell apoptosis were detected at 72 hr post-infection. (A, B) The expression level of BRG1 mRNA and protein was detected by qRT-PCR and western blot and normalized to that of GAPDH and β-actin, respectively. Histogram showed the expression level of BRG1 mRNA (A) and protein (B) in Ad.BRG1-infected and Ad.Null-infected aortic SMCs. (C) Cells were stained with annexin V-FITC and propidium iodide (PI). Flow cytometric contour plots showed the percentage of stained cells. Histogram showed the percentage of Annexin V+/PI- and Annexin V+/PI+ cells of Ad.BRG1-infected and Ad.Null-infected aortic SMCs. Data represent mean ± SEM from 4 independent experiments; **, P <0.01 by t test.