BRG1 expression is upregulated in aortic SMCs of TAD specimens. (A) Representative images of immunohistochemical staining for BRG1 in aortic media of TAD and normal aortic tissues. Expression level of BRG1 protein was moderate in TAD and was weak in normal tissue. Bar =50 μm. (B, C) The expression of BRG1 mRNA and protein in TAD and normal tissues was detected by qRT-PCR and western blot and normalized to that of GAPDH and β-actin, respectively. Each dot represents the relative expression level of BRG1 mRNA (B) and protein (C) of a tissue sample (n =30 for TAD, n =12 for normal tissues) with the line indicating the mean level; **, P <0.01 by paired t test.