Table 1 Primer sequence of TIMP-1, -2, MMP2, MMP9, p38MAPK and GAPDH for real time PCR
Gene name | Primer sequence | |
|---|---|---|
TIMP-1 | Forward | 5′AGACACACCAGAGCAGATACC3′ |
Reverse | 5′CAGCTACAGGCCTTACTGGAA3′ | |
TIMP-2 | Forward | 5′GCTCCAACCCTGTCCTAACC3′ |
Reverse | 5′GCACAACACGAAAATGCCCT3′ | |
MMP2 | Forward | 5′TTTCTATGGCTGCCCCAAGG3′ |
Reverse | 5′GTCAAGGTCACCTGTCTGGG3′ | |
MMP9 | Forward | 5′CGGATCCCCAACCTTTTCCA3′ |
Reverse | 5′GTGCCTGTCACAAAAGCCAG3′ | |
p38 MAPK | Forward | 5′AAGACTCGTTGGAACCCCAG3′ |
Reverse | 5′GGGTCGTGGTACTGAGCAAA3′ | |
GADPH | Forward | 5′CAAGGTCATCCATGACAACTTG3′ |
Reverse | 5′GTCCACCACCCTGTTGCTGTAG3′ |