Figure 5

Validation of miRNA microarray data of selected miRNAs by RT-qPCR. RT-qPCR was performed on 5 differentially expressed miRNAs (3 upregulated: miR-466, miR-574-3p, miR-3613-3p; and 2 downregulated: miR-1, miR-26a-5p) to confirm the microarray data. (A) Illustration of microarray results. Expression levels of the 5 miRNAs in the AF group based on fold changes of signal intensity in the microarray relative to the NSR group. The log₂ ^{(fold change)} values are plotted on the y-axis. (B) Illustration of RT-qPCR results. Using RT-qPCR, the expression levels of each miRNA in the AF group relative to the NRS group was estimated by the comparative cycle threshold 2^-ΔΔCt method. The log₂ ^{(relative expression)} values are plotted on the y-axis. There is a significant difference for each miRNA, consistent with the microarray result.