Adenine nucleotide metabolites in the effluents immediately after reperfusion. After 30 min-ischemia, hearts were reperfused with a normal solution, and effluents were collected every 20 sec for 120 sec. Effluents were also collected at pre-ischemia. Endogenous adenine nucleotide metabolites released from hearts into the effluents were measured by HPLC. Data are means ±S.E.M (n = 10). Ado; adenosine, IN; inosine, HX; hypoxanthine.