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Figure 5 | BMC Cardiovascular Disorders

Figure 5

From: Expression of matrix metalloproteinase-12 in aortic dissection

Figure 5

Effects of different concentration of Ca 2+ ions on MMP-12 activity. The MMP-12 assay was based on the MMP-12 hydrolysis of FRET substrate ( AnaSpec, Inc, Fremont, CA,USA) .A total of 100 μl reaction mixture consisting of 1 μl (100 ng) MMP-12, different concentrations of Ca2+ ions (0-40 mM) and 50 μl of buffer ( AnaSpec, Inc, Fremont, CA,USA)were incubated at 37°C for 10 min. Fluorescence was read at Ex/Em wavelengths = 340 nm/490 nm for 60 min. RFU: Relative Fluorescence Unit.

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