Effects of carvedilol treatment on cardiac cAMP response element binding protein expression and phosphorylation in acute coxsackievirus B3-induced myocarditis

Table 1 Primer sequences used for semi-quantitative RT-PCR

mRNA	Primers	Annealing temperature ( ° C)	Cycles	Product (bp)
CVB3	F-CGGTACCTTTGTGCGCCTGT	61	30	314
CVB3	R-CAGGCCGCCAACGCAGCC	61	30	314
IL-6	F- TGCTGGTGACAACCACGGCC	60	33	308
IL-6	R- GTACTCCAGAAGACCAGAGG	60	33	308
TNF-α	F-CCTGTAGCCCACGTCGTAGC	50	31	374
TNF-α	R-TTGACCTCAGCGCTGAGTTG	50	31	374
MCP-1	F-GCCAACTCTCACTGAAGCC	50	30	161
MCP-1	R-GCTGGTGAATGAGTAGCAGC	50	30	161
β-actin	F-AGGGAAATCGTGCGTGACAT	55	24	450
	R-CATCTGCTGGAAGGTGGACA

ISSN: 1471-2261