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Figure 2 | BMC Cardiovascular Disorders

Figure 2

From: Low levels of IgM antibodies to oxidized cardiolipin increase and high levels decrease risk of cardiovascular disease among 60-year olds: a prospective study

Figure 2

a Competition ELISA of IgM anti-OxCL binding to plated OxCL in the absence or presence of indicated amounts of OxCL or CL preincubated with sera. The results are expressed as Percent inhibition = (OD without competitor - OD with competitor)/ OD without competitor X100. Each value shown is the mean±standard deviation. b ELISA of IgM anti-OxCL or anti-CL which had been postincubated with different concentration of ß2GP1 (for 1 hour at RT). ß2GPI could increase anti-CL binding to CL but not anti-OxCL binding to OxCL. The results are expressed as mean±standard deviation. c Competition ELISA of binding of IgM anti-OxCL, anti-CL and aOxLDL respective antigens after preincubation with the indicated concentrations of oxLDL. The results are expressed as Percent inhibition = (OD without competitor - OD with competitor)/ OD without competitor X100. Each value shown is the mean±standard deviation. d Competition ELISA of binding of IgM anti-OxCL and anti-PC to respective antigens after preincubation with the indicated concentrations of PC-BSA. The results are expressed as Percent inhibition = (OD without competitor - OD with competitor)/ OD without competitor X100. Each value shown is the mean±standard deviation.

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