Figure 1

Automated image analysis. The Image J protocol is illustrated using fibronectin staining in the lesser curvature of PAK HZ and PAK KO mice. Minimal threshold intensity is selected such that only the endothelial cell stained regions are positive. The inner 90 pixels are isolated as the endothelial layer. Consistent area selection across sections gives the mean integrated density in endothelial cells as a function of area and intensity. Image J analysis cartoon of DAB stained regions (red) in the lesser curvature (LC) of PAK heterozygote (HZ) [left] and knockout (KO) mice [right].