level of cytokine (MCP-1, ICAM-1andTNF-α) in myocardial tissue and serum. Blood samples and hearts isolated from C3H/HeN (TLR4 competent) and C3H/HeJ (TLR4 defective) mice were subjected to single injection of trastuzumab (2 mg/Kg i.p). While control hearts were subjecting to normal saline i.p. (A) level of TNF-α (B) level of monocyte chemoattractant protein-1 (MCP-1).(C) ICAM-1 was obtained after harvest heart, Western immunoblotting assay was performed as described in Materials and Methods, markedly reduced ICAM-1 in the He J mutant (TLR4-/-).