Figure 3

Representative cross-sectional tissue slices of hearts stained with picrosirius red show minimal fibrosis in untreated mdx hearts (mean 1% collagen; Panel A) and significantly increased fibrosis in mdx hearts exposed to 0.5 mg/kg/day isoproterenol for 4 weeks (mean 3.0% collagen; Panel B) and in mdx hearts exposed to 0.5 mg/kg/day isoproterenol and treated with P188 for 4 weeks (mean 2.9% collagen; Panel C). Staining is consistent with patchy fibrosis patterns seen in older mdx mice